SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
نویسندگان
چکیده
منابع مشابه
Some Observations on Negatively Stained Mitochondria
A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side of the mitochondrial envelope which faces the matrix. It is attached to the underlying membrane by...
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Recently investigators have reported knob-like particles on mitochondrial membranes isolated from different organisms (1, 2, 4, 5). This report presents observations on the structure of plant mitochondria, negatively stained with phospho-tungstic acid, which show knob-like projections on the membranes. MATERIALS AND METHODS Castor beans (Ricinus communis) were soaked for 24 hours in distilled w...
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The Davson-Daniel l i model of m e m b r a n e structure features a bimolecular leaflet of phospholipids in lamel lar a r rangement , covered on bo th sides by protein (1). This model is in keeping with m a n y electron microscopic and other observations on cellular membranes (2-4) and mixtures of phospholipids and proteins (5-8). An al ternat ive model of m e m b r a n e structure involving a ...
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In experiments carried out with sucrose-isolated rat liver mitochondria, C(14)-sucrose seems to penetrate most of the mitochondrial space rapidly, whereas it seems to penetrate the remainder relatively slowly. However, taking into account the wide size distribution found in isolated preparations, these rates have been found consistent with the kinetics expected from the assumption of a single c...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1963
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.17.2.443